Phis-sumo
Webb11 jan. 2016 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the above sequences into a pHis-SUMO vector (Senturia et al. 2010). Site-directed mutagenesis was performed using the Quickchange method (Agilent). All plasmid sequences were verified by DNA sequencing (Genewiz). Webb18 aug. 2024 · 公司开创了两大独家技术平台:用于细胞信号和药物研发的泛素蛋白酶体系统 (UPS),以及增强目的蛋白在大肠杆菌、酵母、昆虫和哺乳动物细胞中表达与纯化的 SUMO 融合表达系统。作为 LifeSensors 中国区总代理,艾美捷科技为您提供最全面的产品和最优质的服务。
Phis-sumo
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Webb14 apr. 2024 · Wakatakakage (back) beats Midorifuji on the 12th day of the 15-day Spring Grand Sumo Tournament at Edion Arena Osaka in Osaka on March 23, 2024. (Kyodo) ==Kyodo. The popular 28-year-old, who won his first grand tournament in March last year, has held the sport's third-highest rank for seven straight tournaments. WebbShow Static Map. Sticky ends from different BstAPI sites may not be compatible. BssHII is typically used at 50°C, but is 75% active at 37°C. EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
Webb1 mars 2024 · The plasmids pHis-SUMO-me31B, pHis-SUMO-4E1 and pHis-SUMO-4E3 were transformed into BL21-CodonPlus-RIL (Agilent Technologies) for recombinant protein expression. Me31B was present in inclusion bodies and purified in denaturing conditions (50 mM phosphate pH 8.0; 400 mM NaCl; 8 M urea) in Nickel-agarose and eluted in a … WebbpSUMO载体价格2500元,pSUMO载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为卡那,质粒大小为5598bp,是大肠杆菌蛋白表达载体。
http://www.biofeng.com/zaiti/dachang/pSUMO.html WebbpETsumo载体简介. The Champion pET-SUMO Expression System produces the highest levels of soluble protein in E. coli. It utilizes a small ubiquitin-related modifier (SUMO) …
http://www.biofeng.com/zaiti/dachang/pET-SUMO.html avatarkitWebb本实验同时构建了两种高效表达载体phis-sumo-spa(全长蛋白)和phis-sumo-fspa(功能区蛋白),转化入宿主菌rosetta,将spa的全长蛋白和功能区片段与sumo标签进行融合表达,利用sumo标签的作用提高spa蛋白的稳定性和活性,经过iptg诱导和表达条件的摸索,利用akta蛋白纯化系统纯化 ... avataronWebb11 apr. 2024 · Sky Germany’s Florian Plettenberg now has an update, claiming he has had Arsenal’s interest ‘confirmed’. Plettenberg notes that Baku is open to leaving Wolfsburg this summer and his dream ... avatarroku101WebbpHISi载体价格3000元,pHISi载体质粒图谱(Vector map),载体序列(Sequence)见下文。 avatarokiWebbBL21 DE3 E. coli competent cells were transformed with pHis- SUMO- 3ABC and a single transformed colony was grown overnight at 37 °C in LB media supplemented with 50 −1µg ml kanamycin with ... avataron aWebbThe chemokines CCL19 and CCL21 are essential for recruiting CCR7-expressing dendritic cells bearing pathogen-derived antigens and lymphocytes to lymph nodes, where the two cell types meet to launch an adaptive immune response against the invading pathogen. avatarussyWebbPlasmid pET-SUMO-hGSDMD from Dr. Hongbo Luo's lab contains the insert Gasdermin D and is published in Cell Rep. 2024 Mar 13;22(11):2924-2936. doi: … avatarotulos s.l