WebbAn adaptation of the published assay (33) was used. HeLa cells were transfected with Str-KDEL-TNF-SBP-mCherry construct as described above, and 24 hours after transfection, … Webb20 jan. 2024 · Rush assays on hole BBY21069 from one new pegmatite returned 47.75 m (true thickness 10m) of 1.34 % Li 2 O 42 holes have now been completed totalling …
Bi‐allelic mutation in SEC16B alters collagen trafficking and …
WebbIn the RUSH assay, HeLa cells were transfected with plasmids encoding mouse N-terminal Shh fragment with the signal peptide removed (amino acid: 25 to 198) fused downstream of enhanced green fluorescent protein (EGFP) and the streptavidin binding pep- tide (SBP) that had an N-terminal signal peptide derived from IL-2 (SBP-EGFP-ShhN or … Webb29 maj 2024 · Using the RUSH assay, we studied the synchronous secretion of diverse cargos: collagen type X (ColX), VSV-G, secretory soluble EGFP (ssEGFP), gp135 … chris choice
SOKOMAN MINERALS : CUTS TWO HIGH-GRADE INTERVALS AT …
Webb19 jan. 2024 · Identification of a highly elongated tubular organelle through mislocalized DsRed2-ER-5 We initially attempted to deliver the red fluorescent protein (FP) DsRed2 into the ER lumen of COS-7 cells via an N-terminal signal peptide. WebbIn order to monitor local exocytosis of cargos, we describe in this chapter how to perform synchronization of the anterograde transport of an exocytic cargo of interest using the … WebbEach sample preparation step requires controls to be in place; from sample submittal, through preparation and return or disposal of samples. Generating a homogeneous … chris choi chef